Development of a Homogeneous Assay to Measure Remnant Lipoprotein Cholesterol
نویسندگان
چکیده
منابع مشابه
Development of a homogeneous assay to measure remnant lipoprotein cholesterol.
BACKGROUND Quantification of triglyceride-rich lipoprotein (TRL) remnants is useful for risk assessment of coronary artery disease and the diagnosis of type III hyperlipoproteinemia. Although an immunoseparation procedure for remnant-like particle cholesterol has been evaluated extensively in recent years, available methods for measuring TRL remnants have not achieved wide use in routine labora...
متن کاملLimitations of automated remnant lipoprotein cholesterol assay for diagnostic use.
I wish to comment on the limitations of an automated remnant lipoprotein cholesterol (RemL-C) assay reported in Clinical Chemistry (1 ). Remnants are lipoprotein particles produced after newly formed triglyceride-rich lipoproteins (TRLs) of either hepatic or intestinal origin enter the plasma space and undergo lipolysis via the action of lipoprotein lipase in the capillary bed. During this proc...
متن کاملHighly sensitive cholesterol assay with enzymatic cycling applied to measurement of remnant lipoprotein-cholesterol in serum.
BACKGROUND Remnant lipoprotein-cholesterol (RLP-C) concentrations in sera of healthy individuals are very low (0.080-0.437 mmol/L), making conventional cholesterol methods poorly suited to this purpose. We have developed a highly sensitive cholesterol assay (CD method) and applied it to the RLP-C assay. METHODS The CD shuttled cholesterol reversibly between reduced and oxidized forms in the p...
متن کاملHomogeneous assay for direct determination of high-density lipoprotein cholesterol evaluated.
We evaluated a new homogeneous assay for quantifying high-density lipoprotein cholesterol (HDL-C). The assay included four reagents: polyethylene glycol for "wrapping" chylomicrons, very-low-density lipoproteins (VLDL), and low-density lipoproteins (LDL); antibodies specific for apolipoprotein (apo) B and apo C-III to produce aggregates of chylomicrons, VLDL, and LDL; enzymes for the enzymatic ...
متن کاملDevelopment of a homogeneous assay for measurement of small dense LDL cholesterol.
BACKGROUND Plasma concentrations of small dense (sd)-LDL are associated with the prevalence of cardiovascular events. However, the special equipment and long assay times required for sd-LDL measurement have hindered its clinical application. Herein, we report development of a simple homogeneous assay for sd-LDL-cholesterol (C) adaptable to autoanalyzers. MATERIALS AND METHODS We identified su...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2007
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2007.092296